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Fundamentals of Microfluidics and Lab on a Chip for ...

Derived from the highly popular Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery (2006), this volume is also readily adaptable for classroom use. Problem sets in each chapter help students test their assimilation of the material and clarify challenging concepts.

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Isothermal Polymerase Amplification in a Centrifugal ...

implementation of nucleic acid amplification assays in microfluidic platforms. For the first time we present a lab-on-a-chip cartridge designed for the automated amplification of doublestranded DNA based on recombinase polymerase amplification (RPA). Our fluidic cartridge consists of a polymer foil. To demonstrate the potential of the

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MAGNETIC BEAD BASED DNA PURIFICATION ON A .

KEYWORDS: Microfluidic, DNA purification, DNA extraction, Lab-on-a-Chip INTRODUCTION During the last years, automated systems for nucleic acid analysis gained more and more importance e.g. for in-vitro diagnostics. Therefore the "on-disk" integration of DNA purification is crucial for the development of fully integrated

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Lab on a Chip

Lab on a Chip PAPER Cite this: Lab Chip,2019,19,3853 Received 16th August 2019, ... such binding/washing extraction principles onto microfluidic lab-on-a-chip systems.4–8 Recent advances in integration and ... usability of nucleic acid analysis technologies.

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[PDF] Integrated microfluidic pumps and valves operated by ...

DOI: 10.1039/c9lc00422j Corpus ID: 201117842. Integrated microfluidic pumps and valves operated by finger actuation. @article{Park2019IntegratedMP, title={Integrated microfluidic pumps and valves operated by finger actuation.}, author={Juhwan Park and J. Park}, journal={Lab on a chip}, year={2019} }

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Translating Nucleic Acid Amplification Assays to the ...

Title:Translating Nucleic Acid Amplification Assays to the Microscale: Lab on a Chip for Point-of-Care Molecular Diagnostics VOLUME: 12 ISSUE: 5 Author(s):Michael G. Mauk, Jinzhao Song, Yubing Tong, Haim H. Bau and Changchun Liu Affiliation:Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

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(PDF) Pre-storage of gelified reagents in a lab-on-a-foil ...

agents.1 Although many POC devices utilize immunoassays To make the leap from lab-on-a-chip to practical POC involving antigen–antibody binding, nucleic acid-based diag- devices, it is desirable to preload and store all the required nostics, which measure DNA or RNA, are becoming more reagents on chip.11 Such self-contained devices would elim ...

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Point-of-care nucleic acid testing for infectious diseases ...

Nucleic acid testing for infectious diseases at the point of care is beginning to enter clinical practice in developed and developing countries; especially for applications requiring fast turnaround times, and in settings where a centralized laboratory approach faces limitations. Current systems for clinical diagnostic applications are mainly PCR-based, can only be used in hospitals, and are ...

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Variants of PCR - Wikipedia

Hot-start PCR is a technique performed manually by heating the reaction components to the DNA melting temperature (e.g. 95 °C) before adding the polymerase. In this way, non-specific amplification at lower temperatures is prevented. Alternatively, specialized reagents inhibit the polymerase's activity at ambient temperature, either by the binding of an antibody, or by the presence of ...

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Droplet microfluidics for single cell and nucleic acid ...

The aim of this thesis is to develop droplet microfluidics based methods for analysis of single cells and nucleic acids. In Paper I, a method for time-series analysis of mammalian cells, using automated fluorescence microscopy and image analysis technique is presented. The cell-containing droplets were trapped on-chip and imaged

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Lab-on-a-chip solutions designed for being operated on ...

Jan 01, 2010· Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C et al. Microfluidic Lab-on-a-Foil for Nucleic Acid Analysis based on Isothermal Recombinase Polymerase Amplification of DNA. Lab Chip 2009;10:887-93. [6]

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Detection of Streptococcus equi subsp. equi in guttural ...

men arriving at the lab, results can take longer, 1 to 3 days, if specimens need to be shipped to the lab. Streptococcus equi qPCR assays have been developed to detect various target genes: a partial DNA sequence of SeM (fibrinogen-binding protein gene),2,3 seeI (a superantigen-encoding gene),4 eqbE

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[PDF] LabDisk with complete reagent prestorage for sample ...

Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). S. Lutz, P. Weber, +10 authors F. von Stetten Chemistry, Medicine

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Selective local lysis and sampling of live cells for ...

Jul 14, 2016· Download PDF. Introduction. Most ... E. Micro-immunohistochemistry using a microfluidic probe. Lab Chip 12, 1040 (2012). ... local lysis and sampling of live cells for nucleic acid analysis using ...

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[PDF] Integrated microfluidic pumps and valves operated by ...

DOI: 10.1039/c9lc00422j Corpus ID: 201117842. Integrated microfluidic pumps and valves operated by finger actuation. @article{Park2019IntegratedMP, title={Integrated microfluidic pumps and valves operated by finger actuation.}, author={Juhwan Park and J. Park}, journal={Lab on a chip}, year={2019} }

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Miniaturized PCR chips for nucleic acid amplification and ...

The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply 'Lab on a chip' systems, have witnessed steady advances over .

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Automation of Silica Bead-based Nucleic Acid Extraction on ...

Nov 11, 2016· We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber .

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WO2015015175A1 - Fluidic cartridge for nucleic acid ...

A cartridge for assay of a target nucleic acid sequence in a liquid sample. The cartridge comprises: a fluidic portion through which the sample flows and in which nucleic acid amplification and detection takes place; a pneumatic portion which controls flow through the fluidic portion; and at least two electrodes which provide a potential difference for the detection of an amplified nucleic ...

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GMS | GMS Zeitschrift zur Förderung der Qualitätssicherung ...

Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, Müller C, Mark D, Roth G, Munday P, Armes N, Piepenburg O, Zengerle R, von Stetten F. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip. 2010 Apr 7;10(7):887-93. DOI: 10.1039/b921140c 19. Ahmad F, Hashsham SA.

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Future microfluidic and nanofluidic modular platforms for ...

in nucleic acid profiling. Equipment and analysis costs, throughput, and normalization6,7 still represent obstacles for large-scale nucleic acid biomarker validation8–14 and adoption in the clinics15–17 Differences in the nucleic acid purification method, detection technology, and labo-ratory protocol often lead to different profiling ...

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Microfluidic device reads up to four consecutive base ...

May 01, 2004· Nucleic Acids Research, Volume 32, Issue 9, 1 May 2004, ... The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab‐on‐a‐chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications ...

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Nucleic Acid Analysis | Analytical Chemistry

Ultrasensitive electrical detection of nucleic acids by hematin catalysed silver nanoparticle formation in sub-microgapped biosensors. Biosensors and Bioelectronics 2008, 24 (4), 787-791.

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Utility of lab‐on‐a‐chip technology for high‐throughput ...

Sep 30, 2005· On‐chip electrophoresis can provide size separations of nucleic acids and proteins similar to more traditional slab gel electrophoresis. Lab‐on‐a‐chip (LoaC) systems utilize on‐chip electrophoresis in conjunction with sizing calibration, sensitive detection schemes, and sophisticated data analysis to achieve rapid analysis times (< 120 s).

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(PDF) Microfluidic-Based Nucleic Acid Amplification ...

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology. ... the Lab Disk is mounted to a custom-made holder [71]. ... particularly for nucleic acid (NA) analysis in.

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[PDF] Microfluidic-Based Nucleic Acid Amplification ...

Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices ...

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From single-molecule detection to next-generation ...

2013) and single-cell analysis (Brouzes et al. 2009). In the current review, we aim to survey recent devel-opments in the use of droplet-based microfluidics for nucleic acid analysis, first highlighting key areas where such microfluidic tools have had significant effect and .

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A lab-in-a-foil microfluidic reactor based on phaseguiding ...

Feb 05, 2018· A microfluidic lab in a foil chip for whole genome amplification of genomic DNA is proposed. • The proposed device has no moving parts and is based on phaseguiding. • Phaseguiding is achieved by an array of pillars. • The DNA amplified by multiple displacement amplification has low contamination as shown by sequencing.

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(PDF) Diffusion-based microfluidic PCR for "one-pot ...

Diffusion-based microfluidic PCR for "one-pot" analysis of cells. 6 Pages. ... some type of isolation step to remove the lysis reagent and undesired intracellular molecules while preserving nucleic acids. This is typically done by adsorption of nucleic acids on solid surfaces (e.g. beads or matrices) while replacing the solution. 2,3,17,18 ...

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Journal of Controlled Release - MITCHELL LAB

Jun 10, 2019· interact with the target can be identified by their b-DNA using deep sequencing [41]. This b-DNA concept has recently been applied to LNP delivery in the context of identifying barcoded NPs that target tumors [39] as well as those that deliver nucleic acid therapeutics to various organs in vivo[40,42].

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(PDF) A droplet microfluidic approach to single-stream ...

In light of this situation, recent development in lab-on-chip systems has demonstrated miniaturized platforms capable of isolating nucleic acids and amplifying biomarkers of interest using polymerase chain reaction (PCR) (Pal et al. 2005;Easley et al. 2006;Zhang et al. 2010).

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Lutz, S.; Microfluidic lab-on-a-foil for nucleic acid analysis

Lutz, S.; Microfluidic lab-on-a-foil for nucleic acid analysis 2010-04 Microfluidic lab-on-a-foil for RPA @ Lab Chip.pdf — PDF document, 262Kb

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